Effects of Kiperin Elixea, a multicomponent antioxidant supplement, on redox homeostasis and longevity-associated gene expression in human epithelial cell models.

Abstract

Aging is characterized by declining NAD + levels, mitochondrial dysfunction and disruption of redox homeostasis. Multi-component formulations targeting interconnected hallmarks of aging have emerged as potential geroprotective strategies. We evaluated the molecular and functional effects of Elixea, an NMN (nicotinamide mononucleotide)-based formulation containing NAD + precursors (NMN, NADH), mitochondrial cofactors (CoQ10, PQQ), and antioxidants (resveratrol, glutathione), in human epithelial cell models. hTERT-HME1 and HaCaT cells were treated with 0.1 μg/mL Elixea for 24 h. Cellular viability, regenerative kinetics, total antioxidant status (TAS), total oxidant status (TOS), and expression of longevity-associated genes were assessed. Elixea maintained normal cell viability and migration dynamics, supporting the preservation of basal epithelial function. In hTERT-HME1 cells, treatment significantly improved redox homeostasis, as evidenced by increased TAS and reduced TOS levels. Gene expression analysis revealed significant upregulation of IL10, FOXO3A, APOE, GPX1 , and FOXO1A , indicating activation of pathways associated with antioxidant defense, anti-inflammatory signaling, and metabolic regulation. In HaCaT cells, responses were more selective, including upregulation of APOE, FOXO3A , and RUVBL1 . Collectively, these findings suggest that Elixea modulates conserved longevity-associated pathways in a cell type-dependent manner, promoting cellular resilience without evidence of cytotoxicity or overt stress-associated responses. This study provides mechanistic evidence supporting the geroprotective potential of multi-component interventions targeting redox and metabolic networks in epithelial systems.